=Paper=
{{Paper
|id=Vol-1498/HAICTA_2015_paper45
|storemode=property
|title=Body Amino Acid Composition of Axillary seabream (Pagellus acarne R., 1827) Catched from Dardanelles (Canakkale, Turkey)
|pdfUrl=https://ceur-ws.org/Vol-1498/HAICTA_2015_paper45.pdf
|volume=Vol-1498
|dblpUrl=https://dblp.org/rec/conf/haicta/OztekinKASYHE15
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==Body Amino Acid Composition of Axillary seabream (Pagellus acarne R., 1827) Catched from Dardanelles (Canakkale, Turkey)==
https://ceur-ws.org/Vol-1498/HAICTA_2015_paper45.pdf
Body Amino Acid Composition of Axillary seabream
(Pagellus acarne R., 1827) Catched from Dardanelles
(Canakkale, Turkey)
Alkan Öztekin1, Bayram Kızılkaya2, Alparslan Aslan3, Kahraman Selvi4, Sevdan
Yılmaz2, Olcay Hisar5, Sebahattin Ergün2
1
Department of Fisheries Engineering, Faculty of Marine Sciences and Technology, Çanakkale
Onsekiz Mart University, Turkey, e-mail: alkanoztekin@comu.edu.tr
2
Department of Aquaculture, Faculty of Marine Sciences and Technology, Çanakkale Onsekiz
Mart University, Turkey
3
Department of Marine Technology Engineering, Faculty of Marine Sciences and Technology,
Çanakkale Onsekiz Mart University, Turkey
4
Yenice Vocational Collage, Çanakkale Onsekiz Mart University, Turkey
5
Department of Basic Science, Faculty of Marine Sciences and Technology, Çanakkale
Onsekiz Mart University, Turkey
Abstract. Amino acid composition of the whole body tissue of Axillary
seabream (Pagellus acarne) was determined. Fish was catched from
Dardanelles (April 2013) via fishing line. The most abundant amino acid was
Glutamic Acid (11.0 g/100 g crude protein) and Valine was the most abundant
essential amino acid (6.2 g/100 g crude protein). The total essential amino acid
content was 32.3 g/100 g crude protein and the total nonessential amino acid
content was 42.5 g/100 g crude protein.
Keywords: Pagellus acarne, Axillary seabream, Dardanelles, Amino acid
composition
1 Introduction
Axillary seabream is a sparid distributed in Eastern Atlantic and Mediterranean Sea,
occurring from Bay of Biscay to Senegal, Cap Vert, the Azores, Madeira and the
Canary Islands (AO CECAF Scientific Committee (AO CECAF Scientific
Committee, 2007). This species is found on hard and sandy bottoms at depths
ranging from 0 to 500 m, mainly between 40 - 100 m (Muus and Nielsen 1999;
Bauchot and Hureau 1990). It is an hermaphrodite, omnivorous species, but prefer
worms, mollusks and small crustaceans (Bauchot and Hureau 1990).
Fish is a important source of essential amino acids with growing evidence of
potential benefits and fish consumption relate to the use of proteins of high biological
value, as well as certain minerals and vitamins (Shaviklo et al 2015). On the other
hand, the fish growth optimization is closely linked to the supply of dietary protein
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and it can be maximized by manipulating the composition of the dietary amino acid
(Limin et al. 2006).
Although several fish species are cultured worldwide, data on the quantitative
requirements for all the essential amino acids have been reported for only a limited
number of fish (NRC, 1993). Axillary seabream is considered promising new fish
species for marine aquaculture in Turkey (Guner et al 2013). However, its amino
acid composition unknown.
The aim of the present study was to determine amino acid composition in
territorially consumed Axillary seabream in Dardanelles.
2 Material and Method
Axillary seabream was caught from sampling station (Güzelyalı) in April 2013 in
Dardanelles using a fishing line. All samples were collected in the daytime and
stored in ice baths until they arrived in laboratory. The samples (n=10) were dried at
105 °C in an oven until they were of constant weight before use.
In order to determine amino acid profiles, the dried samples were hydrolyzed at
110oC for 24 hours with 6.0 M hydrochloric acid. After evaporation, hydrolyzed
samples were all dissolved in citrate–sodium citrate buffer (0.1 M, pH 2.2) (Chi al.
2008).
High performance liquid chromatography (HPLC) is generally used for the
analysis of amino acid contents. O-Phthalaldehyde (OPA) is the important pre-
column derivatization reagent by HPLC-Floresans Dedection or UV. The column
and flow rate is a C18 reversed phase and 1.2 mL/min. Fluorimetrical detection of
OPA derivatives is carried out at an Ex. 350 nm, Em. 450 nm for OPA derivatization.
Mobile Phase consistent of phosphate buffer solution (pH 7.3, 0,02 M) (A) and
ACN: MeOH: water (45:45:10, v/v/v) (B). The gradient elution system (A/B) is used
as 90/10-3 min, 90/10-10 min,78/22-2 min, 72/28-9 min, 70/30-4 min, 35/65-7 min,
25/75-1 min, 0/100-1 min, 0/100-2min, 90/10-2 min. The derivatization reagents are
10 μL 3-Mercaptopropionic Acid in 10 mL Borate Buffer and 10 mg O-
Phthalaldehyde in 5 mL Borate Buffer. Firstly, it is added 45 µL MPA + 25 µL + 10
µL sample at the derivatization procedure, and then mixed and waited for 1 min.
Then, it is added 10 µL FMOC in the solution, and mixed and waited for 2 min.
Finally, 10 µL of derivatizated amino acids is injected to HPLC.
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3 Results
The amino acid data are expressed as g/100 g amino acids in Table 1. The most
abundant amino acid was Glutamic Acid (11.0 g/100 g crude protein) and Valine was
the most abundant essential amino acid (6.2 g/100 g crude protein). The total
essential amino acid content was 32.3 g/100 g crude protein and the total
nonessential amino acid content was 42.5 g/100 g crude protein.
4 Discussion
It is widely recognized that the specific requirement for amino acids should be
determined in terms of optimum amount of dietary protein necessary for most
efficient animal production (Kim and Lall 2000).
Table 1. Whole body amino acids (g/100 g crude protein) of Axillary seabream on dry weight
Essential Amino Acids (EAA)
Histidine (HIS) 2.1±0.1
Isoleucine (ILE) 6.1±0.3
Leucine (LEU) 2.5±0.2
Lysine (LYS)4 1.1±0.4
Methionine (MET) 4.5±0.1
Phenylalanine (PHE) 5.1±0.2
Threonine (THR) 4.7±0.1
Valine (VAL) 6.2±0.1
Total 32.3
Nonessential Amino Acids (NEAA)
Alanine (ALA) 6.4±0.1
Aspartic Acid (ASP) 8.4±0.1
Glutamic Acid (GLU) 11.0±0.4
Glycine (GLY) 4.9±0.2
Serine (SER) 4.4±0.1
Tyrosine (TYR) 2.1±0.1
Hydroxylysine (HLY) 1.1±0.1
Cystine (C-C) 4.2±0.2
Total 42.5
The results of present study show that Axillary seabream has a high content of
Valine and Isoleucine which are essential amino acids. Valine is involved in many
metabolic pathways and is considered indispensable for protein synthesis and
optimal fish growth (Abidi and Khan 2004). In addition, isoleucine is a branched-
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chain, amino acid needed in the body to produce certain biochemical compounds that
help in energy production and together with the other two branched-chain amino
acids promotes tissue building (Khan and Abidi 2007). In the present study, glutamic
acid which is essential for cell proliferation was the most predominant among all the
amino acids in Axillary seabream.
This study demonstrate that Axillary seabream is good source of amino acids.
Acknowledgments. Authors would like to thank Cahit Ceviz, Osman Odabaşı and
Umut Tuncer for their assistance.
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