<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Archiving and Interchange DTD v1.0 20120330//EN" "JATS-archivearticle1.dtd">
<article xmlns:xlink="http://www.w3.org/1999/xlink">
  <front>
    <journal-meta />
    <article-meta>
      <title-group>
        <article-title>Classification of Winter Rapeseed Cultivars and their Yield Characters with the Common Vector Approach</article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author">
          <string-name>Nurdilek Gülmezoğlu</string-name>
          <xref ref-type="aff" rid="aff2">2</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Zehra Aytaç</string-name>
          <email>zehrak@ogu.edu.tr</email>
          <xref ref-type="aff" rid="aff1">1</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>M. Bilginer Gülmezoğlu</string-name>
          <xref ref-type="aff" rid="aff0">0</xref>
        </contrib>
        <aff id="aff0">
          <label>0</label>
          <institution>Department of Electrical and Electronics Engineering, Faculty of Engineering and Architecture, Eskişehir Osmangazi University</institution>
          ,
          <addr-line>26480, Eskişehir</addr-line>
          ,
          <country country="TR">Turkey</country>
        </aff>
        <aff id="aff1">
          <label>1</label>
          <institution>Department of Field Crops, Faculty of Agriculture, Eskişehir Osmangazi University</institution>
          ,
          <addr-line>26480, Eskşehir</addr-line>
          ,
          <country country="TR">Turkey</country>
        </aff>
        <aff id="aff2">
          <label>2</label>
          <institution>Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Eskişehir Osmangazi University</institution>
          ,
          <addr-line>26480, Eskişehir</addr-line>
          ,
          <country country="TR">Turkey</country>
        </aff>
      </contrib-group>
      <fpage>713</fpage>
      <lpage>719</lpage>
      <abstract>
        <p>In this study, five cultivars (Ceres, Zorro, Falcon, Express, and Samourai) of winter rapeseed were classified by using the common vector approach (CVA). For this purpose, seven yield characters (plant height, number of branches per plant, number of pods per plant, number of pods on main stem, number of seeds per pod, pod length and thousand seed weight) of each cultivar were used. The seven and six yield characters taken from five cultivars were classified by using CVA. 100% classification rate is guaranteed for the training set of both studies. For the test set, the classification of five cultivars has low performance, but the classification of seven and six yield characters gave satisfactory results. It is concluded that the CVA method was successful in the classification of different varieties belonging to any plant and/or of different characters belonging to any variety.</p>
      </abstract>
      <kwd-group>
        <kwd>Character classification</kwd>
        <kwd>common vector approach</kwd>
        <kwd>rapeseed classification</kwd>
      </kwd-group>
    </article-meta>
  </front>
  <body>
    <sec id="sec-1">
      <title>-</title>
      <p>
        1 Introduction
Rapeseed is an important oilseed crop in the agricultural systems of many arid and
semiarid areas. Agronomic and quality advantages of new varieties have enlarged
their production areas worldwide (Gül et al. 2007). Rapeseed in Turkey is mostly
cultivated as a winter annual for oil production and rarely livestock feed. If planted in
spring, they can be grown as summer crop but the seed yield would be decreased due
to short growing season and lack of enough water at the end of growing season, thus,
winter cropping is preferred. The canola cultivars are slow growing especially in
winter and most of them will complete their life cycle in 210 to 270 days
        <xref ref-type="bibr" rid="ref10">(Sharghi et
al. 2011)</xref>
        . There are wide variations among the cultivated canola cultivars with
respect to seed and oil yields per unit area at different planting dates as well as
irrigation regimes. The seed yield and maturity of canola is greatly influenced by
fertility management, seeding rate and seeding date
        <xref ref-type="bibr" rid="ref3">(Grant and Bailey, 1993)</xref>
        .
      </p>
      <p>
        Computer-based algorithms have been extensively used in agriculture in order to
classify various plants and their characters or samples. Classification of plant
varieties with computer algorithms has been become popular in recent years. The
common vectors representing the invariant features of the plants can be extracted by
eliminating the differences in each class of plants (Gülmezoglu 1999). Then these
common vectors are used for the classification of varieties and characters of plants.
Different methods were used in order to derive features or parameters from plant
varieties
        <xref ref-type="bibr" rid="ref11 ref12 ref13 ref14 ref2 ref9">(Wang et al., 1999; Zayas et al., 1996; Utku and Köksel, 1998; Delwiche &amp;
Massie, 1996; Neuman and Bushuk, 1987; Shuaib et al., 2010)</xref>
        . Some classifications
were analyzed for characters of rapeseed. Ali et al. (2012) analyzed for near infrared
spectroscopy and principal component grain of rapeseed. Jankulovska et al. (2014)
presented the use of different multivariate approaches to classify rapeseed genotypes
based on quantitative traits. Some of these parameters were plant height, number of
primary branches per plant, number of pods per plant, pod length, number of seeds
per pod, seed weight per pod, 1000 seed weight, seed weight per plant and oil
content. This model has been applied in agricultural sciences to identify the effect of
yield character differences (Gülmezoğlu and Gülmezoğlu 2015). However, there is
no information on the use in plant breeding programs.
      </p>
      <p>In this study, we considered five cultivars (Ceres, Zorro, Falcon, Express, and
Samourai) of winter rapeseed. Initially, these cultivars were classified by using the
common vector approach (CVA). Secondly, seven yield characters (plant height,
number of branches per plant, number of pods per plant, number of pods on main
stem, number of seeds per pod, pod length and thousand seed weight) and six yield
characters excepting number of branches per plant were classified by using CVA.
2</p>
    </sec>
    <sec id="sec-2">
      <title>Material and Method</title>
      <p>This research was carried out over three years during 2005 at the Faculty of
Agriculture of Eskisehir Osmangazi University, Eskisehir (39o 48′ N; 30o 31′ E; 789
m in elevation). The field experiments included five winter rapeseed cultivars (Ceres,
Zorro, Falcon, Express, Synergy and Samourai). The experiment was planned in a
Randomized Complete Block Design with four replications. The individual plots
were 3 m long and consisted of five rows. The cultivars were sown on the first week
of September, using a seed rate of 10 kg ha -1 in 40 cm spaced lines on a well
prepared seed bed. The experiment was fertilized respectively with 150 kg N ha -1 as
ammonium nitrate: 33-0-0 and 50 kg P2O5 ha -1 as di-ammonium phosphate:
18-460. The plants were irrigated once during emergence and thinned at the rosette stage.
The weeds were controlled by hand weeding.</p>
      <p>Each cultivar was represented with seven yield characters which are plant height,
number of branches per plant, number of pods per plant, number of pods on main
stem, number of seeds per pod, pod length and thousand seed weight. Each character
includes 20 plant samples which were taken from field study conducted during
growing year.</p>
      <p>As in all classification methods, CVA has training and testing stages. In the
training stage, a common vector which represents common or invariant properties of
each class is calculated and an in difference subspace for each class is constructed.
Let the vectors a1c,ac2,...,acm Є Rn be the feature vectors for a variety-class C in the
training set where m ≤ n. Then each of these feature vectors which are assumed to be
linearly independent can be written as
aic = aic,dif + accom +ε ic for i=1,2, …, m (1)
c
where the vector ai,dif indicates the differences resulting from climatic effects and
c
alien-pollination, and the vector acom is the common vector of the variety or
c
character class C, and ε i represents the error vector (Gülmezoğlu et al. 2001). The
common vector can be obtained from the subspace method. Let us define the
covariance matrix of the feature vectors belonging to a variety or character class as
m
i=1
Φ =
∑ (aic − aave )(aic − aave )
c c</p>
      <p>T
c
where aave is the average feature vector of Cth class whose covariance matrix is to
be calculated and T indicates the transpose of a matrix.</p>
      <p>The eigenvalues of the covariance matrix Ф are non-negative and they can be
written in decreasing order: λ1 ≥ λ2 ≥ K ≥ λn. Let u1c, uc2,..., ucn be the orthonormal
eigenvectors corresponding to these eigenvalues. The first (m-1) eigenvectors of the
covariance matrix corresponding to the nonzero eigenvalues form an orthonormal
basis for the difference subspace B (Gülmezoğlu et al. 2001). The orthogonal
complement, B┴, is spanned by all the eigenvectors corresponding to the zero
eigenvalues. This subspace is called the indifference subspace and has a dimension
of (n-m+1). The direct sum of two subspaces B and B┴ is the whole space, and the
intersection of them is the null space. The common vector can be shown as the linear
combination of the eigenvectors corresponding to the zero eigenvalues of Ф
(Gülmezoğlu et al. 2001), that is,</p>
      <p>accom = aic , ucm ucm +L + aic , ucn ucn ∀ i=1,2,…,m (3)
From here, the common vector acom is the projection of any feature vector onto
the indifference subspace B┴. The common vector represents the common properties
or invariant features of the variety or character class C. The common vector is
independent from index i. Therefore, the common vector is unique for each class and
c
all the error vectors ε i would be zero.</p>
      <p>During the classification stage, the following decision criterion is used:
2
dis tan ce = argmin
1≤C≤S
n</p>
      <p>⎧⎪⎡ c ⎤ c ⎪⎫
∑ ⎨⎢(ax - aic )T u j ⎥ u j ⎬
j=m ⎩⎪⎣ ⎦ ⎭⎪
(2)
(4)
where ax is an unknown or test vector and S indicates the total number of classes. If
the distance is minimum for any class C, the feature vector ax is assigned to class
C.</p>
      <p>Classification algorithm given above can be summarized as follows:
Step 1: Construct feature vectors by using samples taken for each character
belonging to any cultivar. Be sure that number of samples in each feature vector (or
dimension of each feature vector) is greater than number of feature vectors (or
characters) for each cultivar.</p>
      <p>Step 2: Find the covariance matrix (Eq. (2)) for each cultivar by using feature vectors
belonging to that cultivar.</p>
      <p>Step 3: Find the eigenvalues λi and corresponding eigenvectors ui for each
covariance matrix.</p>
      <p>Step 4: Find the common vector (Eq. (3)) for each cultivar by using the (n-m+1)
eigenvectors corresponding to zero eigenvalues.</p>
      <p>Step 5: When an unknown feature vector ax is given, classify this vector by using
Eq. (4).
3</p>
    </sec>
    <sec id="sec-3">
      <title>Results</title>
      <p>In the first study, each of five cultivars forms one class in the CVA method. Seven
characters each of which includes 20 samples for each cultivar or class form seven
feature vectors of that class. Therefore, there are five classes and each class has seven
feature vectors. When the feature vectors (characters) used in the training stage were
tested, all classes (cultivars) were correctly classified, i.e., 100% correct recognition
rate was obtained. When the “leave-one-out” strategy was used in the testing stage,
that is, when six feature characters were used in the training stage and remaining one
character was tested, 25.7% correct recognition rate was obtained as average of
“leave-one-out” steps. The results obtained from this study are given in Table 1. The
average score obtained in the test set is very low because samples included in the
characters representing different cultivars are very close to each other.</p>
      <p>In the second study, the characters were classified by using CVA. First of all,
seven characters were considered and each of seven characters forms one class in the
CVA method. Twenty samples taken from each cultivar for any character form one
feature vector of that character. Therefore, there are seven classes and each class has
five feature vectors. When the feature vectors, each of them includes 20 samples,
used in the training stage were tested, all classes (characters) were correctly
classified, i.e., 100% correct recognition rate was obtained. When the
“leave-oneout” strategy was used in the testing stage, that is, when six feature characters were
used in the training stage and remaining one character was tested, 77.1% correct
recognition rate was obtained as average of “leave-one-out” steps. The results
obtained for this study are given in Table 2.
Varieties
Samourai
Zorro
Falcon
Ceres
Express
Average
Yield Characters
Plant Height
Number of Branches per Plant
Number of Pods per Plant
Number of Pods on Main Stem
Number of Seeds per Pod
Pod Length
Thousand Seed Weight
Average</p>
      <p>Secondly, six characters excepting number of branches per plant were classified.
All characters were correctly classified (100% correct recognition rate was obtained)
in the training set and 90% recognition rate was obtained for the test set. These
scores are remarkable because samples taken from cultivars for each character are
close to each other and well represent that character. These results are given in Table
3.</p>
      <p>
        It is known that varieties of different plants have been successfully classified by
using various computer-based algorithms. Especially, classification of wheat
varieties with computer algorithms has been become popular in recent years (Zayas
et al. 1996, Utku and Köksel 1998, Neuman and Bushuk 198
        <xref ref-type="bibr" rid="ref7">7, Gülmezoğlu and
Gülmezoğlu 2015</xref>
        ). Therefore, in this study, first of all, five rapeseed varieties were
classified by using CVA method. In spite of 100% correct recognition rate in the
training set, very low recognition rate (25.7%) was obtained in the test set. The
reason is that samples included in the characters representing different varieties are
very close to each other. Thus, common properties or invariant features of each
variety cannot be correctly extracted and indifference subspace cannot be constructed
efficiently.
      </p>
      <p>Additionally, characters were classified by using CVA method. Initially, seven
characters are applied to the classification process and 100% and 77.1% recognition
rates were obtained for the training and test sets respectively. The reason of low
score for the test set is that the samples belonging to number of branches per plant
character are similar to samples belonging to other characters. When the number of
branches per plant character is discarded, that is, when the remaining six characters
are classified, 90% recognition rate is obtained for the test set.</p>
    </sec>
    <sec id="sec-4">
      <title>5 Conclusion</title>
      <p>It is concluded that the CVA method was very successful in the classification of
different varieties belonging to any plant and/or of different characters belonging to
any variety. Such classifications can be very helpful in assignment of unknown
varieties or unknown characters to identify plant. When more specific characters are
extracted for each variety of plants, good performance can be achieved from the
classification process.</p>
      <p>As a future work, number of varieties for any plant and the number of characters
will be increased. Satisfactory results are also expected from this work.</p>
    </sec>
  </body>
  <back>
    <ref-list>
      <ref id="ref1">
        <mixed-citation>
          1.
          <string-name>
            <surname>Ali</surname>
            ,
            <given-names>I.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Shah</surname>
            ,
            <given-names>S.A.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Ahmed</surname>
            ,
            <given-names>H.M.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Rehman</surname>
            <given-names>K.U.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Ahmad</surname>
            ,
            <given-names>M.</given-names>
          </string-name>
          (
          <year>2012</year>
          )
          <article-title>Studies On Genetic Diversity For Seed Quality In Rapeseed (Brassica Napus L.) Germplasm of Pakistan Through Near Infrared Spectroscopy And Principal Component Analysis</article-title>
          .
          <source>Pak. J. Bot.</source>
          ,
          <volume>44</volume>
          :
          <fpage>219</fpage>
          -
          <lpage>222</lpage>
          , Special Issue March
          <year>2012</year>
          .
        </mixed-citation>
      </ref>
      <ref id="ref2">
        <mixed-citation>
          2.
          <string-name>
            <surname>Delwiche</surname>
            ,
            <given-names>S.R.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Massie</surname>
            ,
            <given-names>D.R.</given-names>
          </string-name>
          (
          <year>1996</year>
          )
          <article-title>Classification of wheat by visible and nearinfrared reflectance from single kernels</article-title>
          .
          <source>Analytical Techniques and Instrumentation</source>
          <volume>73</volume>
          (
          <issue>3</issue>
          )
          <fpage>399</fpage>
          -
          <lpage>405</lpage>
          .
        </mixed-citation>
      </ref>
      <ref id="ref3">
        <mixed-citation>
          3.
          <string-name>
            <surname>Grant</surname>
            ,
            <given-names>C.A.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Bailey</surname>
            ,
            <given-names>L.D.</given-names>
          </string-name>
          (
          <year>1993</year>
          )
          <article-title>Fertility management in canola production</article-title>
          .
          <source>Canadian Journal of Plant Science</source>
          <volume>73</volume>
          :
          <fpage>651</fpage>
          -
          <lpage>670</lpage>
          .
        </mixed-citation>
      </ref>
      <ref id="ref4">
        <mixed-citation>
          4.
          <string-name>
            <surname>Gül</surname>
            ,
            <given-names>M.K.</given-names>
          </string-name>
          <string-name>
            <surname>Egesel</surname>
            ,
            <given-names>C.Ö.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Kahriman</surname>
            ,
            <given-names>F.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Tayyar</surname>
          </string-name>
          , Ş. (
          <year>2007</year>
          )
          <article-title>Investigation of Some Seed Quality Components in Winter Rapeseed Grown in Çanakkale Province</article-title>
          . Akdeniz University Journal of the Faculty of Agriculture.,
          <year>2007</year>
          ,
          <volume>20</volume>
          (
          <issue>1</issue>
          ),
          <fpage>87</fpage>
          -
          <lpage>92</lpage>
          .
        </mixed-citation>
      </ref>
      <ref id="ref5">
        <mixed-citation>
          5.
          <string-name>
            <surname>Gülmezoğlu</surname>
            <given-names>M.B.</given-names>
          </string-name>
          , (
          <year>1999</year>
          ),
          <article-title>“A Novel Approach to Isolated Word Recognition”</article-title>
          ,
          <source>IEEE Trans. Speech and Audio Processing</source>
          , vol.
          <volume>7</volume>
          , No.
          <issue>6</issue>
          , pp.
          <fpage>620</fpage>
          -
          <lpage>628</lpage>
          .
        </mixed-citation>
      </ref>
      <ref id="ref6">
        <mixed-citation>
          6.
          <string-name>
            <surname>Gülmezoğlu</surname>
            ,
            <given-names>M.B.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Dzhafarov</surname>
            ,
            <given-names>V.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Barkana</surname>
            ,
            <given-names>A.</given-names>
          </string-name>
          (
          <year>2001</year>
          )
          <article-title>The Common Vector approach and its Relation to Principal Component Analysis</article-title>
          .
          <source>IEEE Trans. Speech and Audio Processing</source>
          <volume>9</volume>
          (
          <issue>6</issue>
          ),
          <fpage>655</fpage>
          -
          <lpage>662</lpage>
          .
        </mixed-citation>
      </ref>
      <ref id="ref7">
        <mixed-citation>
          7.
          <string-name>
            <surname>Gülmezoğlu</surname>
            ,
            <given-names>M.B.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Gülmezoğlu</surname>
            ,
            <given-names>N.</given-names>
          </string-name>
          (
          <year>2015</year>
          )
          <article-title>Classification of bread wheat varieties and their yield characters with the common vector approach</article-title>
          . International Conference on Chemical,
          <article-title>Environmental and Biological Sciences (CEBS-</article-title>
          <year>2015</year>
          ),
          <year>March</year>
          ,
          <fpage>18</fpage>
          -
          <lpage>19</lpage>
          ,
          <year>2015</year>
          , Dubai, BAE.
        </mixed-citation>
      </ref>
      <ref id="ref8">
        <mixed-citation>
          8.
          <string-name>
            <surname>Jankulovska</surname>
            ,
            <given-names>M.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Ivanovska</surname>
            ,
            <given-names>S.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Marjanovic-Jeromela</surname>
            ,
            <given-names>A.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Bolaric</surname>
            ,
            <given-names>S.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Jankuloski</surname>
            ,
            <given-names>L.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Dimov</surname>
            ,
            <given-names>Z</given-names>
          </string-name>
          , Bosev,
          <string-name>
            <given-names>D.</given-names>
            ,
            <surname>Kuzmanovska</surname>
          </string-name>
          ,
          <string-name>
            <surname>B.</surname>
          </string-name>
          (
          <year>2014</year>
          )
          <article-title>Multivariate Analysis Of Uantitative Traits Can Effectively Classify Rapeseed Germplasm</article-title>
          .
          <source>Genetika</source>
          , Vol.
          <volume>46</volume>
          , No.
          <volume>2</volume>
          ,
          <fpage>545</fpage>
          -
          <lpage>559</lpage>
          .
        </mixed-citation>
      </ref>
      <ref id="ref9">
        <mixed-citation>
          9.
          <string-name>
            <surname>Neuman</surname>
            <given-names>M.</given-names>
          </string-name>
          and
          <string-name>
            <surname>Bushuk</surname>
            ,
            <given-names>W.</given-names>
          </string-name>
          (
          <year>1987</year>
          )
          <article-title>Discrimination of wheat class and variety by digital image analysis of whole grain samples</article-title>
          .
          <source>Journal of Cereal Science</source>
          <volume>6</volume>
          (
          <issue>2</issue>
          )
          <fpage>125</fpage>
          -
          <lpage>132</lpage>
          .
        </mixed-citation>
      </ref>
      <ref id="ref10">
        <mixed-citation>
          10.
          <string-name>
            <surname>Sharghi</surname>
            ,
            <given-names>S.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Shirani</surname>
            ,
            <given-names>A.M.R.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Noormohammadi</surname>
            , G. Zahedi,
            <given-names>H.</given-names>
          </string-name>
          (
          <year>2011</year>
          )
          <article-title>Yield and yield components of six canola (Brassica napus L.) cultivars affected by planting date and water deficit stress</article-title>
          .
          <source>African Journal of Biotechnology</source>
          .
          <volume>10</volume>
          (
          <issue>46</issue>
          ):
          <fpage>9309</fpage>
          -
          <lpage>9313</lpage>
          .
        </mixed-citation>
      </ref>
      <ref id="ref11">
        <mixed-citation>
          11.
          <string-name>
            <surname>Shuaib</surname>
            ,
            <given-names>M</given-names>
          </string-name>
          , Jamal,
          <string-name>
            <given-names>M.</given-names>
            ,
            <surname>Akbar</surname>
          </string-name>
          ,
          <string-name>
            <given-names>H.</given-names>
            ,
            <surname>Khan</surname>
          </string-name>
          ,
          <string-name>
            <given-names>I.</given-names>
            ,
            <surname>Khalid</surname>
          </string-name>
          ,
          <string-name>
            <surname>R.</surname>
          </string-name>
          (
          <year>2010</year>
          )
          <article-title>Evaluation of wheat by polyacrylamide gel electrophoresis</article-title>
          .
          <source>African Journal of Biotechnology</source>
          <volume>9</volume>
          (
          <issue>2</issue>
          )
          <fpage>243</fpage>
          -
          <lpage>247</lpage>
          .
        </mixed-citation>
      </ref>
      <ref id="ref12">
        <mixed-citation>
          12.
          <string-name>
            <surname>Utku</surname>
            ,
            <given-names>H.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Köksel</surname>
            ,
            <given-names>H.</given-names>
          </string-name>
          (
          <year>1998</year>
          )
          <article-title>Use of statistical filters in the classification of wheats by image analysis</article-title>
          .
          <source>Journal of Food Engineering</source>
          <volume>36</volume>
          (
          <issue>4</issue>
          )
          <fpage>385</fpage>
          -
          <lpage>394</lpage>
          .
        </mixed-citation>
      </ref>
      <ref id="ref13">
        <mixed-citation>
          13.
          <string-name>
            <surname>Wang</surname>
            ,
            <given-names>D.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Dowell</surname>
            <given-names>F. E.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Lacey</surname>
            ,
            <given-names>R. E.</given-names>
          </string-name>
          (
          <year>1999</year>
          )
          <article-title>Single wheat kernel color classification using neural networks</article-title>
          .
          <source>Transactions of the ASABE</source>
          <volume>42</volume>
          (
          <issue>1</issue>
          )
          <fpage>233</fpage>
          -
          <lpage>240</lpage>
          .
        </mixed-citation>
      </ref>
      <ref id="ref14">
        <mixed-citation>
          14.
          <string-name>
            <surname>Zayas</surname>
            ,
            <given-names>I. Y.</given-names>
          </string-name>
          <string-name>
            <surname>Martin</surname>
            ,
            <given-names>C. R. Steele J. L.</given-names>
          </string-name>
          ,
          <string-name>
            <surname>Katsevich</surname>
            ,
            <given-names>A.</given-names>
          </string-name>
          (
          <year>1996</year>
          )
          <article-title>Wheat classification using image analysis and crush-force parameters</article-title>
          .
          <source>Transactions of the ASABE</source>
          <volume>39</volume>
          (
          <issue>6</issue>
          )
          <fpage>2199</fpage>
          -
          <lpage>2204</lpage>
          . eligible
        </mixed-citation>
      </ref>
    </ref-list>
  </back>
</article>