=Paper=
{{Paper
|id=Vol-2030/HAICTA_2017_paper51
|storemode=property
|title=Contamination of Winter Wheat with Fusarium Mycotoxin Depending on Environment Conditions and Cultivar
|pdfUrl=https://ceur-ws.org/Vol-2030/HAICTA_2017_paper51.pdf
|volume=Vol-2030
|authors=Grazyna Podolska,Edyta Boguszewska
|dblpUrl=https://dblp.org/rec/conf/haicta/PodolskaB17
}}
==Contamination of Winter Wheat with Fusarium Mycotoxin Depending on Environment Conditions and Cultivar==
https://ceur-ws.org/Vol-2030/HAICTA_2017_paper51.pdf
Contamination of winter wheat with Fusarium
mycotoxin depending on environment conditions and
cultivar
Grażyna Podolska, Edyta Boguszewska
Department of Cereal Crop Production, Institute of Soil Science and Plant Cultivation- State
Research Institute, Pulawy, Poland, e-mail: grazyna.podolska@iung.pulawy.pl
Abstract. The objectives was to investigate relationship between weather
conditions, cultivar and deoxynivalenol (DON) concentration in winter wheat.
Concentrations of DON were analyzed in 10 winter wheat cultivars: Batuta,
Ostroga, Markiza, Nateja, Muszelka, Tonacja, Izyda, Satyna, Fregata, Kohelia.
The winter wheat cultivars were naturally contaminated and collected from
different locations of Poland, i.e. Srem Wojtostwo (φ = 52o05´, λ = 17o02),
Radostowo (φ = 53o59´, λ = 18o45), Czeslawice (φ = 51o19´, λ = 22o16´).
Grain samples were analyzed for contamination with deoxynivalenol (DON)
by the enzyme-linked immunosorbent analysis (ELISA) method. The cultivar
and weather conditions, as well as field locations strongly modified the
concentration of deoxynivalenol in grains. The samples of Tonacja, Ostroga
and Muszelka cultivars showed higher contamination with DON, but the
lowest Nateja, Kohelia, Batuta cv. The precipitation in May, June and July
strongly affected seed contamination. More rain contributed to increased wheat
contamination with DON.
Keywords: winter wheat, cultivar, mycotoxin, deoxynivalenol, weather
conditions, morphological features.
1 Introduction
The occurrence of mycotoxins in various crops is a food safety issue of a great
concern worldwide. Mycotoxins that are frequently found in cereals are secondary
metabolites produced mainly by Fusarium ear blight pathogens, which are common
in the temperate climatic zone of Europe, America, and Asia (Creppy et al. 2002).
None of the geographical regions is free from the occurrence of mycotoxins.
Contamination with these compounds affects 25 percent of the world crops annually.
Economic costs incurred by crop producers due to mycotoxin contamination of the
grains of cereals, oilseeds, and feed are estimated at nearly billion dollars a year. The
particularly dangerous and commonly occurring is deoxynivalenol (DON) mainly
produced by Fusarium culmorum and Fusarium graminearum (Bryden, 2012,
Creppy et al. 2002, Hajšlová et al. 2007, Marin et al. 2013).
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The occurrence of Fusarium mycotoxins, mainly DON, in cereal grains as well as
in various cereal based foodstuffs has been reported in several studies. The report
(Schothorst and Egmond 2004) provides an overview of Fusarium toxin levels in
crops harvested in 12 European countries (Austria, Belgium, Denmark, Finland,
France, Germany, Italy, the Netherlands, Norway, Portugal, Sweden, the United
Kingdom). DON was found in many samples. Similarly, the survey presented by
Joint FAO/WHO Expert Committee on Food Additives (JECFA 2001) showed that
DON was the most abundant mycotoxin in cereals, regardless of the country they
were harvested in. In southwest Germany, the presence of DON in wheat flour was
detected in 98% samples. DON content was in the range from 15 to 965 µg kg-1 in
white flour, and from 15 to 1379 µg kg-1 in wholegrain flour (Schollenberger et al.
2002). Also in Poland, the cyclical contamination of cereal grains with DON had
been observed. (Cegielska-Radziejewska et al. 2009). The incidence of mycotoxins
can vary from year to year depending on many factors such as weather conditions
and/or agricultural practices (Obst et al., 2000). Edwards (2004), Czaban et al.
(2015), Mikos and Podolska (2013) reported that besides weather conditions, a
cultivar had an important role in mycotoxin contamination of cereal kernels. The
objective of this research was to investigate relationship between weather,
environment conditions, cultivar and DON concentration in winter wheat.
2 Materials and methods
2.1 Field experiments
The experiment was carried out in the years of 2009-2011 in 3 Experimental
Stations located in different regions of Poland: Srem Wojtostwo (φ = 52o05´, λ =
17o02´) , Radostowo (φ = 53o59´, λ = 18o45´), Czeslawice (φ = 51o19´, λ = 22o16´).
The experiment involved 10 winter wheat cultivars: Batuta, Ostroga, Markiza,
Nateja, Muszelka, Tonacja, Izyda, Satyna, Fregata, Kohelia. The cultivars differed
from each other in terms of heading, wax coating on head, plant height, stem pith,
density of spike, length of spike, arista, length of arista, width of lemma, shape of
lemma, length of lemma. The crop managements (sowing term, sowing, density,
fertilization, crop protection were on the same level in each experimental stations).
The winter wheat was sown on very good (Radostowo) and good wheat soil
complexes (Srem Wojtostwo, Czeslawice). The winter wheat was harvested in full
maturity phase. In each experimental stations, during vegetation period of winter
wheat, weather conditions (temperature, precipitation) were monitored. Grain
samples were analyzed for DON contamination with toxins. Preparation of the grain
samples and ELISA test procedure for determination of contents of deoxynivalenol
(DON) in the grain were carried out according to Cegielska-Radziejewska et al.
(2009), Mikos-Szymanska and Podolska (2013), Czaban et al. (2015).
Analysis of mycotoxin contents: a quantitative analysis of DON was carried out
using enzyme-linked immunosorbent analysis (ELISA) commercial kit (Neogen
Corporation, Food Safety Diagnostics: Veratox® for DON 5/5 – 8331NE). The
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method is based on the antibody-antigen interaction and has been approved by the
AOAC Research Institute (Certificate No. 950702).
2.2 Statistical analysis:
Statistica 6.0 software (StatSoft Inc.) was used for statistical analysis. The
compatibility of variables with normal distribution was tested using the Shapiro-Wilk
test. Verification of the hypothesis was performed using nonparametric (Kruskal-
Wallis test). Assumed significance level = 0.05.
3 Results
The applied treatments (year, location and cultivar) modified the winter wheat
mycotoxins contamination by DON.
Concentration of mycotoxin in winter wheat seeds significantly depended on the
vegetation season (years). In all years, the investigated mycotoxins were detected,
but at different levels. The highest DON concentration was observed in 2011 year,
lower in 2010, but the lowest in 2009 (Table 1). The concentration of DON in winter
wheat seeds from Srem Wojtostwo was 10,0 µg kg−1, from Radostowo 146,7 µg kg−1,
while from Czeslawice 273,3 µg kg−1 (Table 1).
In the present research, a high correlation between concentration of mycotoxin and
precipitation has been found (Table 2). The occurrence of DON was similarly
affected by rainfall in May (r = 0.89819), June (r = 0.80220), July (r = 0.84016). The
number of DON was not significantly correlated with temperature in May, June and
July (Table. 2).
Table 1. DON contamination (µg kg−1) of winter wheat seeds depending on the years and
localization
Year 2009 2010 2011 Śrem Radostowo Czesławice
Wojtostwo
DON 86, 7 106,33 256, 7 10,0 146,7 273,3
Table 2. Correlation coefficient and simple regression between DON contamination of winter
wheat seeds and precipitation and temperature (data from 2009-2011).
Month Precipitation Temperature
Correlation Correlation Correlation Correlatio
coefficient n coefficient
May =-0,3255+0,01379x r= 0,89919 = 1,5746 - 0,0962x r= -0,3926
June =-0,3139+0,01045x r= 0,80220 = 0,66670 - ,0216x r= -0,0946
July =-0,2721+0,00396x r= 0,84016 = 0,42167 – 0,080x r= -0,0176
bold-significant correlation
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The contamination of winter wheat cultivars in 2011 was shown in Figure 1. There
were significant differences cultivars in terms of DON contamination in 2011. The
concentration of DON ranged from 200 µg kg−1 (Nateja cv.) to 1400 µg kg−1
(Tonacja cv.). Significantly the lowest concentration of DON was recorded for the
seeds Nateja cv. compared with Tonacja, Muszelka and Fregata. The concentration
of DON was significantly lower in the seeds of Nateja, Satyna and Batuta.
Figure 1. Concentration of DON in winter wheat cultivars (2011 year).
In order to find correlation between morphological characteristics of winter wheat
cultivars and mycotoxin contamination, eleven features were taken into consideration
There was a significant negative correlation between plant height and stem pith.
Shorter cultivars with thin stem pith were the most contaminated. (Table 3).
Table 3. Correlation coefficient between morphological characteristics of winter wheat
cultivars and mycotoxin contamination
1 2 3 4 5 6 7 8 9 10 11
-0.04 0.39 -0.71 -0.58 0.12 0.48 0.18 0.17 0.47 0.48 0.24
bold- significant correlation
(1-term of heading, 2-wax coating on head, 3-plant high, 4-stem pith, 5-dense of
spike, 6-length of spike, 7-arista, 8-length of arista, 9-with of lemma, 10-shape of
lemma, 11-length of lemma).
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4 Discussion and Conclusions
Research conducted in numerous countries has shown that grain contamination by
Fusarium and mycotoxin level were affected mainly by weather conditions (Bryla et
al. 2016). Moderate temperatures (15-30°C) combined with prolonged periods of
high humidity during the blossoming and/or earing phases promote the accumulation
of DON and are the best indicators of fusariosis (Hooker et al..2002, Cowger et al.
2009.). Bernhoft et al. (2012) reported that low temperature before harvest increased
DON concentration in wheat grain. Favourable conditions – high precipitations and
relatively high temperatures during the period when the wheat is most susceptible to
infection (i.e. from flowering to the soft dough stage of kernel development)
(Edwards 2004, Obst et al. 2000 Hajslova et al., 2007) were obviously factors
responsible for the high mycotoxin levels. It has been confirmed in the present study.
We found a high correlation between concentration DON and precipitation in May,
June and July. More rain contributed to increased wheat contamination with DON.
We also found that besides weather conditions, cultivars play important role in
DON concentration in winter wheat. The significantly lowest concentration of DON
was noted in the seeds of Nateja, Kohelia, Batuta cv. compared with Tonacja,
Ostroga and Muszelka. Similar results were obtained by Bryla et al. (2016). Among
10 cultivars, Fidelius cv. was the least contaminated with DON, while Bamberka
Forkida and Kampana, the most (Bryla et al. 2016). Bai et al. (2001) who conducted
research in artificial inoculation of winter wheat by F. graminearum, proved
significant differences in DON level among cultivars. Out of 116 varieties, they
selected 16 with a small amount of DON. They found that these varieties may be
useful as sources for breeding wheat cultivars with lower DON level. They also
found that cultivars with scabbed spikelets and scabbed kernels had less level in
DON. In our study we found that shorter winter wheat cultivars and cultivars with
thin stem pith were the most contaminated with DON.
Our studies have confirmed a significant impact of weather on the amount of
DON in wheat kernels, and indicated that, under conditions conducive to the
formation of toxins, there are varieties that accumulate small amounts of mycotoxins.
Such varieties should be recommended for cultivation as safe for health reasons.
References
1. Bai, G-H. Plattner, R. Desjardins, A. Kolb, F. (2001) Resistance to Fusarium
head blight and deoxynivalenol accumulation in wheat. Plant Breeding 120,
p.1-6.
2. Bernhoft, A. Torp, M. Clasen, P. E. Løes, A. K. Kristoffersen, A.B. (2012).
Influence of agronomic and climatic factors on Fusarium infestation and
mycotoxin contamination of cereals in Norway. Food Additives and
Contaminants part A 29: 1129-1140.
3. Bryden, W. L. (2012). Mycotoxin contamination of the feed supply chain:
Implications for animal productivity and feed security. Animal Feed Science
and Technology 173, p. 134–158.
426
4. Bryła, M., Waśkiewicz, A., Podolska, G., Szymczyk, K., Jędrzejczak, R.,
Damaziak, K. and Sułek, A. (2016) Occurrence of 26 mycotoxins in the grain of
cereals cultivated in Poland. Toxins, 8, p. 160.
5. Cegielska-Radziejewska, R., Szablewski, T., Karolczak, K., Kaczmarek, A.,
Kijowski, J. (2009). An immunoenzymatic method for the determination of
mycotoxins contents in cereals and feeds. Nauka Przyroda Technologia 3, p.1-9.
6. Cowger, C.; Patton-Özkurt, J.; Brown-Guedira, G., Perugini, L. (2009)
Postanthesis moisture increased Fusarium head blight and deoxynivalenol levels
in North Carolina winter wheat. Phytopathology, 99, p. 320-327.
7. Creppy, E.E. 2002. Update of survey, regulation and toxic effects of
mycotoxins in Europe. Toxicology Letters, 127, p.19-28.
8. Czaban, J.; Wróblewska, B.; Sułek, A.; Mikos, M.; Boguszewska, E. and
Podolska, G.; Nieróbca, A. (2015). Colonisation of winter wheat grain by
Fusarium spp. and mycotoxin content as dependent on a wheat variety, crop
rotation, a crop management system and weather conditions. Food Additives
and Contaminants part A 32, p. 874–910.
9. Edwards, S.G., 2004. Influence of agricultural practices on Fusarium infestation
of cereals and subsequent contamination of grain by trichothecene mycotoxins.
Toxicology Letters, 153, p. 29–35.
10. Hajšlova, J., Lancova, K., Sehnalova, M., Krplova, A., Zachariašova, M.,
Moravcova, H., Nedělnik, J.,Markova, J. and Ehrenbergerova, J. (2007).
Occurrence of trichothecene mycotoxins in cereals harvested in the Czech
Republic. Czech J. Food. Sci., 25, p. 339–350.
11. Hooker, D.C.; Schaafsma, A.W.; Tamburic-Ilincic, L. (2002) Using Weather
Variables Pre- and Post-heading to Predict Deoxynivalenol Content in Winter
Wheat. Plant Dis. , 86, p. 611–619.
12. Marin, S., Ramos, A.J., Cano-Sancho, G. and Sanchis, V. (2013). Mycotoxins:
Occurrence, toxicology, and exposure assessment. Food and Chemical
Toxicology 60, p. 218–237.
13. Mikos-Szymanska, M. and Podolska, G. (2013) Contamination with Fusarium
mycotoxins in triticale depends on agrotechnical factors and cultivar. Journal of
Phytopathology 151, p. 665–668.
14. Obst, A., Lepschy, J., Beck, R., Bauer, G. and Bechtel, A. (2000) The risk of
toxins by Fusarium graminearum in wheat – interactions between weather and
agronomic factors. Mycotoxin Research, 16, p. 16–20.
15. Schollenberger, M., Jara, H.-T., Suchy, S., Drochner, W. and Muller, H.-M.
(2002). Fusarium toxin in wheat flour collected in an area in southwest
Germany. International Journal of Food Microbiology, 72, p. 85–89.
16. Schothorst, R.C. and Egmond H.P (2004). Report from SCOOP task 3.2.10
“collection of occurrence data of Fusarium toxins in food and assessment of
dietary intake by the population of EU member states”: Subtask: trichothecenes.
Toxicology Letters, vol. 153, p. 133-143
427