Studies on the parameters of ecological adaptability of spring barley varieties in the conditions of Primorsky krai were carried out in 20152018 in the Federal State Budget Scientific Institution “Federal Scientific Center of agribiotechnology in the Far East named after A. K. Chaika”. New mathematical approaches to solving the problems of selection of parent pairs for crossing, which included selected varieties-sources with high grain weight per plant with ecological plasticity, were considered: Erofey, Harkovsky111, Krinichny, Runis and other. As a result of the released ecologically plastic variety samples, a new source material was developed (Primorsky 153 (Primorsky 44 x Patty), Primorsky 167 (Primorsky 5097 x K-19907 x Runis), Primorsky 184 (Primorsky 5021 x Krinichny), Primorsky 212 (Primorsky 44 x Keystone), which is studied in all parts of the breeding process. The most adaptive variety for Primorsky Krai is Primorsky 184 (Primorets) in 2018, it was transferred to the State Variety Testing.
The work was performed in field and laboratory conditions at the Laboratory of breeding of grain and cereal crops
at FSBSI "Federal scientific center of agrobiotechnology in the Far East named after A. K. Chaika" in 2015-2018.
The objects of the study were six varieties of the competitive variety testing, developed with the participation of
source varieties identified by the parameters of adaptability [
Parameters of ecological plasticity of the studied varieties were evaluated using methodics of S. A. Eberhart and
W. A. Russell in the presentation of V. A. Zykina [
The stability index (SI), an indicator of stability level of the variety (ISLV) –according to E. D. Nettevich [
ИС = xcp / V % (1) Variability is considered to be: a) insignificant if V<10 % b) average, if V = 10...20 % с) significant if V > 20 % where υ is the average kwadro deviation, xopt is a maximum value of the trait, x lim is a minimum value of the trait, xcp is an average value of the trait. xopt is a maximum value of the trait, x lim is a minimum value of the trait, xcp is an average value of the trait. 3
S
X ПУСС = Хcp ИС V =
, Sc = xср xlim ,
In breeding work at FSBSI “Federal scientific center of agrobiotechnology in the Far East named after A. K.
Chaika” the main method of development a new source material for spring barley is intraspecific hybridization
followed by individual selection. The parent forms were selected for the adaptive properties and the
ecologicalgeographical principle by mathematical analysis of quantitative traits, which provide direction in the selection. Into
the crossing there were included defined varieties-sources with high grain weight per plant with ecological plasticity:
Yerofey, Radical, Nutans 642 and Acha (Russia), Kharkovsky 111 (Ukraine), Gonar (Republic of Belarus), Krinichny
(Republic of Belarus), Colter and Orest (USA), Samson, Keystone and Kimberly (Canada), Runis (Mongolia), Patty
(France), Marecu, Dorina and Nebi (Germany) [
As a result of the selection process with the participation of the studied source varieties the most productive ones were identified: Primorsky153 (Primorsky 44 x Patty), Primorsky 167(Primorsky5097 x-19907 x Runis), Primorsky 184 (Primorsky 5021 x Krinichny), Primorsky 212 (Primorsky 44 x Keystone) which developed high yield in during three years, providing an addition to the standard at the level of 10-20 %.
In the analysis of the obtained lines, which were studied in 2015-2018 in the competitive test, the main economically valuable features were taken into account: productive tillering, ear length, plant height, number of grains per ear, weight of 1000 grains, weight of grain per plant.
The highest yield and its constituent elements were obtained from Primorskiy 184 and Primorskiy 153 varieties, which have a true excess compared to the Primorskiy 98 standard by 1.8 t/ha (Table 1). Variety Primorsky 153 (22.8 pcs.) stood out by the ear grain content.
The adaptability of varieties to environmental conditions, first of all, is judged by the plasticity and stability of
their yield, as the most important quantitative trait. Evaluation of samples by parameters of stability and plasticity is
possible by studying them in sharply contrasting environmental conditions for several years, which is especially
important in Primorsky krai, where during the vegetation period the frequent weather changes limit realization of the
potential productivity of varieties, and plants are largely exposed to adverse vegetation conditions, which indicates a
wide range of variation, both productivity and other quantitative traits over the years [
The stability variance of the trait shows how reliably the variety corresponds to the plasticity which was estimated by the regression coefficient calculated by the formula (6).
bi = X y I j ,
I 2j where Xy Ij is the sum of the yield results i-th variety for the j-th year by the corresponding value of the index of environmental conditions; I 2j is the sum of the squares of the index of environmental conditions. , e u l a v g in c d S e e r
B 1.9 2.9 2.9 3.3 3.1 2.7 1.6 Calculation of the variance of stability (S2d) were calculated according to the formula in several stages: 1) First, they find the values of the theoretical yield:
X j = X + bi I j 2) Then, determine the deviation of the actual yield from the theoretical formula: 3)
Next, we calculate the variance of the yields stability: where i2 j is the sum of the squares of deviations of the actual yield from the theoretical; n is the number of years.
Studies have shown that intensive varieties include: Primorsky 153 (bi=1,3), Primorsky184 (bi=1,2), Pacific (bi=1,6) and East (bi=1,7) (Table 2). They respond well to the improvement of growing conditions.
To varieties with reduced responsiveness to environmental conditions for yield refers Primorsky 212 (bi=0,8; S2di=1,9). It is characterized by the lowest regression coefficient (bi), reliably different from 1 into the smaller side. It responds poorly to changes of environmental factors.
The level of resistance of varieties to stressful growing conditions reflects the difference between the minimum and maximum yield of Ymin-max , which has a negative sign. The smaller is the gap between the minimum and maximum crop productivity, the higher is the stress resistance of the variety and the wider is the range of its adaptive capabilities. The higher is the breeding value, the more valuable is the variety, and these varieties have high rates of breeding value.
The most important indicators of adaptive properties are the regression coefficients of yields on the indices of environmental conditions. During the years of the study, the most favorable conditions during the growing season were 2015 and 2018, where Ii (the environment index) was 0.8 and 0.2, respectively. The index of environmental conditions was calculated using the formula: X i j −
X i j
I j = lАlВ
In 2016, there were unfavorable conditions caused by heavy precipitation (389 mm) and the minimum temperature during the growing season, which indicates a negative value (I i= -0.9). The intersection of the average productivity in the test, which coefficient regression is equal 1, with the yield ordinate which was recovered from the point with the index of environmental conditions, which is always equal to zero , puts the average – 3.8 t/ha (Fig. 1). The regression lines of yield of Primorskiy 184, Primorskiy 153 and Primors kiy 167 varieties are higher than the average point of the test, which is expressed by a higher level of harvest on average over the years of research.
In general, the comparison of regression coefficients and stability variance allows to characterize varieties by their response to changing environmental conditions and helps in the selection of varieties for different levels of agricultural production. As a result of researches in the competitive test there were defined the grades which possess high plasticity and stability: Primorsky 153 (Primorsky 44 x Patty) and Primorsky184 (Primorsky5021 x Krinichny).
The zoned varieties Tihookeansky and Vostochny, are characterized by high plasticity, present good results of grain harvest in the conditions of monsoon climate in Primorsky Krai. The varieties can form grain yield more than 4 t/ha in the production conditions.
One of the directions in breeding work with barley is development of varieties of brewing direction. To meet the needs of the brewing industry in the region is largely possible through the production of malting barley in the region. In solving this important problem, the primary role belongs to the variety that is able to form high-quality grain in the appropriate conditions of cultivation.
The most valuable in terms of brewing are large fractions of grain, and the best are malting barley which have a mass of 1000 grains from 37,0 to 48,0 g. The weight of 1000 grains of spring barley varieties ranged from 35.2g. of Vostochny variety to 43,9 g. of Tihookeansky variety. The research showed that the protein content in grain samples of spring barley in the competitive variety testing varied from 9,8 g. of variety Vostochny to 11.6 g. in variety Primorsky184 and Primorsky212, the starch content varied from 55,2 g. in the variety Primorsky167 to 62,5 g. in the variety Primorsky 98 (Table 3). Filminess of the varieties was in the range of 6.1 to 9.0 %.
Conclusion ̅ Х
As a result of calculating experiments, the method S. Eberhart and W. Russell [
Thus, evaluation of the adaptive capacity and stability of genotypes by methods of mathematical analysis increases the efficiency of the final stage of selection, reduces the possibility of errors in the selection of varieties for transferring them into the State testing. The above results of the use of modern statistical methods indicate the wide possibilities of their use in breeding. As a result of the parameters of adaptability, there were defined varieties by a set of indicators, they are as follows: Primorskiy 153 and Primorskiy 184 (Primorets). In 2018 Primorets was transferred to the State Commission of the Russian Federation for testing and protection of breeding achievements. 53.4 52.6 54.4 54.9 55.7 59.3 54.5 ̅ Х