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  <front>
    <journal-meta />
    <article-meta>
      <title-group>
        <article-title>A Spatial Interpolation Approach for Environmental Flow Assessment in Bulgarian-Greek Rhodope Mountain Range</article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author">
          <string-name>Ekaterina Ivanova</string-name>
          <email>ivanovae@spase.bas.bg</email>
          <xref ref-type="aff" rid="aff1">1</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Dimitrios Myronidis</string-name>
          <email>myronid@for.auth.gr</email>
          <xref ref-type="aff" rid="aff0">0</xref>
        </contrib>
        <aff id="aff0">
          <label>0</label>
          <institution>School of Forestry and Natural Environment, Aristotle University of Thessaloniki</institution>
          ,
          <addr-line>54124 Thessaloniki</addr-line>
          ,
          <country country="GR">Greece</country>
        </aff>
        <aff id="aff1">
          <label>1</label>
          <institution>Space Research and Technology Institute-Bulgarian Academy of Sciences</institution>
          ,
          <country country="BG">Bulgaria</country>
        </aff>
      </contrib-group>
      <fpage>274</fpage>
      <lpage>285</lpage>
      <abstract>
        <p>Nowadays, the environmental flow (e-flow) is globally recognized as an essential component of the sustainable water resources management. Therefore, defining flow requirements is an important step forward, especially in transboundary regions where different water management practices exist. This study aims to test a spatial interpolation approach for estimating the e-flow in the Bulgarian-Greek Rhodope Mountain Range, incorporating hydrological methods, GIS techniques and expert judgment. It was found that the minimum flow required to maintain rivers and riverine ecosystems in the region with a probability of exceeding 90% of the time ranges from 0.027 to 6.11 m3/s, which represents from 1.92 to 32.98 percentages of the mean annual flow. The base flow variability index varies from 2 (low) to 20 (extremely high). Based on the Tennant method and low-flow duration indices, the rivers were regionalized into 5 ecological management classes that identify the quality of ecosystems and their conservation status.</p>
      </abstract>
      <kwd-group>
        <kwd>Environmental flow</kwd>
        <kwd>Rhodope</kwd>
        <kwd>GIS</kwd>
        <kwd>river ecosystem</kwd>
      </kwd-group>
    </article-meta>
  </front>
  <body>
    <sec id="sec-1">
      <title>1 Introduction</title>
      <p>
        The concept of environmental flow (e-flow) is nowadays recognized as an essential
step towards sustainable management of the natural resources, the need of which is
constantly increasing for the demand to ensure the human livelihoods in the context of
global climate change and growing exigency. This concept, widespread in the last 3
decades, defines e-flow as the flow regime (i.e. quantity, quality, and timing of water
flow) required to sustain freshwater and riverine ecosystems
        <xref ref-type="bibr" rid="ref1 ref10 ref15 ref16 ref18 ref3">(Clausen and Biggs,
2000; EC-Guidance No 31, 2015; Acreman, 2016; Karakoyun et al., 2018; Palmer and
Ruhi, 2019)</xref>
        .
      </p>
      <p>
        One of the main reasons for increasing the use of water is to generate electricity via
hydroelectric power plants (HPPs)
        <xref ref-type="bibr" rid="ref10">(Karakoyun et al., 2018)</xref>
        . As a consequence of the
abundant water reserves of the Rhodope Mountain, a significant part of Bulgaria's
hydropower plants is located here, whereat many of the largest country’s dams have
been constructed during the 50s and 60s of the 20th century. The construction of small
hydroelectric plants (SHP) is also a strategic goal for Greece, which can reduce the
electricity imports and contribute to balance of payments
        <xref ref-type="bibr" rid="ref12">(Myronidis et al., 2008)</xref>
        .
Even supporting the economic development of the countries, the HPPs cause damage
to biological diversity in rivers and their ecosystems, changing the basic components
of the river flow (i.e. volume and timing), as well as the natural interrelation between
the river and its flood areas
        <xref ref-type="bibr" rid="ref10">(Karakoyun et al., 2018)</xref>
        . Degradation of watershed
ecosystems can also affect soil erosion, since the healthy vegetation is one of the best
protections against erosion. Myronidis et al., (2010) address the issue of land
degradation caused by excessive erosion in the Mediterranean region, pointing out the
need of sustainable plan to mitigate the negative impact on natural ecosystems.
      </p>
      <p>
        In the past, many of these environmental problems have not been taken into account
in the watershed management and the construction of HPPs, which in turn requires a
scientific approach to ensure the future sustainable development of the
BulgarianGreek transboundary region. Recently, the rapid development of information
technology has significantly increased the technical capacity to assess the variability
of flow regimes in both temporal and spatial perspective, and hence the variability in
ecosystem processes, using a broad range of spatial scales, resolutions and data
availability
        <xref ref-type="bibr" rid="ref22 ref23 ref25">(Tharme, 2003; Smakhtin et al., 2006)</xref>
        .
      </p>
      <p>This study aims to test a spatial interpolation approach to reveal the spatiotemporal
variability in the flow regime in the Bulgarian-Greek Rhodope Mountain Range,
integrating Geographic Information Systems (GIS) and the most commonly used
hydrological indices that might predict the magnitude, frequency and timing of flow
events to further define environmental flow requirements.
2</p>
    </sec>
    <sec id="sec-2">
      <title>Methods</title>
      <sec id="sec-2-1">
        <title>2.1 Study area and data</title>
        <p>
          The entire territory of The Rhodope Mountain Range was considered in this study,
extending on an approximately 23 500 km2 between longitudes 23o40’E and 26o40’E
and between latitudes 40o50’N and 42o15’N in Bulgarian-Greek transboundary region
(Figure 1). Both the humid continental climate of the North and the Mediterranean
climate of the South influence the local climate of the region. The average annual
temperature varies from 5 to 10-13 °C whilst average annual precipitation ranges
between 600-1100 mm
          <xref ref-type="bibr" rid="ref28">(Yordanova et al., 2002)</xref>
          . The relief differs from
lowmountainous in the south-east to high-mountainous in the west (0–2191 m) with mean
elevation 630 m.
        </p>
        <p>
          The Rhodope Mountain Range is famous for the highest species diversity in the
Balkans and rivers play a significant role in their conservation
          <xref ref-type="bibr" rid="ref26">(Tsiftsis and Tsiripidis,
2012)</xref>
          . According to the Bulgarian Ministry of Environment and Water and the Greek
Ministry of Reconstruction, Environment and Energy the region includes 36 Natura
2000 protected sites (17 in Bulgaria and 19 in Greece) with a total area of 11 000 km2.
The flow inventory data, employed in a previous study
          <xref ref-type="bibr" rid="ref14">(Myronidis and Ivanova, 2020)</xref>
          ,
contain monthly records for maximum, minimum and mean discharges in m3/s for
time period of at least 10 years of measurements (between 1936 and 1995) for 22
pristine watersheds with mean annual discharge from 0.19 to 27.7 m3/s.
        </p>
      </sec>
      <sec id="sec-2-2">
        <title>Environmental flow assessment methodology</title>
        <p>
          A large number of methods have been used for environmental flow assessment,
varying from simple statistics to complex models. Generally, they have been
categorized in Hydrological, Hydraulic Rating, Habitat Simulation and Holistic
methods
          <xref ref-type="bibr" rid="ref10 ref8">(Gopal, 2013; Karakoyun et al., 2018)</xref>
          . In this study simple methodology was
compiled in order to assess environmental flow and classify flow regime for water
management and habitat maintenance. The methodology includes following steps:
1. Selecting hydrological indices that adequately characterize flow regime;
2. Statistical analysis of the hydrological data to arrive at index values;
3. Spatial interpolation of the index values to characterize the spatial variability
of flow regime;
4. Compiling a classification of the flow regime to predetermine the state of
riverine ecosystems, based on sustainable flow requirements, in terms of
Ecological Management Classes (EMC) proposed by the South African DWAF
(1997).
        </p>
        <p>
          The hydrological methods, selected and applied in a historical record, are the most
widely used method of Tennant (1976), based on percentages of mean annual
discharge, and Flow Duration Curve (FDC) method, which is a cumulative frequency
curve representing the percentage of time during which the flow rate is equal or
exceeds particular value
          <xref ref-type="bibr" rid="ref8">(Gopal, 2013)</xref>
          . Several low-flow indices were obtained from
FDCs in a monthly step in order to determine e-flow, including Q50, Q90 and Q95
(daily flows exceeding 50%, 90% and 95% of the time) expressed in both m3/s and %
of mean annual flow (MAF). In addition, Low Exceedance Flow Index – LEFI
(Q90/Q50)
          <xref ref-type="bibr" rid="ref15 ref16 ref19 ref3">(Pyrce, 2004; Clausen and Biggs, 2000)</xref>
          and Baseflow Variability Index –
BVI (Q50/Q90)
          <xref ref-type="bibr" rid="ref17 ref19">(Nelms et al., 1997; Pyrce, 2004)</xref>
          were calculated.
        </p>
        <p>
          Spatial interpolation was applied to all indices to determine flow regime
requirements in their spatial and temporal variability, enforcing “Topo to Raster” tools
in the ArcGIS software, which represents an interpolation technique based on the
ANUDEM program, specially designed to create a surface closer to natural drainage
surface
          <xref ref-type="bibr" rid="ref9">(Hutchinson et al., 2011)</xref>
          . The procedure uses interpolation methods, such as
inverse distance weighted (IDW) interpolation, without losing the surface continuity.
        </p>
        <p>Finally, a holistic approach based on calculated indices was applied to classify rivers
according to certain requirements for maintaining the whole riverine ecosystem in its
ecological integrity.
3</p>
      </sec>
    </sec>
    <sec id="sec-3">
      <title>Results</title>
      <sec id="sec-3-1">
        <title>3.1 Instream flow regime based on the Tennant method</title>
        <p>
          Since the development of Tennant's hydrological methodologies
          <xref ref-type="bibr" rid="ref24">(Tennant, 1976)</xref>
          involves the collection of field habitat, hydraulic and biological data, this method
differs from many others and is considered one of the most suitable for e-flow
assessment
          <xref ref-type="bibr" rid="ref19 ref25">(Tharme, 2003; Pyrce, 2004)</xref>
          . The methodology consists of linking certain
percentages of mean annual flow (MAF) to eight categories of river condition on a
seasonal basis to sustain fish, wildlife, recreation, and related environmental resources.
To apply this method, the mean monthly flow was obtained for all gauging stations in
the Rhodope Mountain Range, averaging the mean monthly discharge data for all
observed years. Then, the percentage of the MAF was calculated month by month
(Table 1).
        </p>
        <p>Once obtained, these percentages were interpolated via ArcGIS software to reveal
the spatial and temporal variability of in-stream flow regimens with respect to different
aquatic and riverine habitat conditions. Following the Tennant’s environmental flow
recommendations, we assumed the threshold of 10% of the MAF as the lowest limit
corresponding to “severe degradation” of a riverine ecosystem. The category up to
10% (max to 30%) of MAF (Apr.–Sept.) was assumed to be “poor”. The other
categories are “fair”, “good”, ”excellent”, “outstanding”, “optimum” and “flushing”,
joined to ranges of 10%–20% (Oct.-Mar.) – 30%–40% (Apr.-Sept.); 20%–30%
(Oct.Mar.) – 40%–50% (Apr.-Sept.); 30%–40% (Oct.-Mar.) – 50%–60% (Apr.-Sept.);
40%–100% (Oct.-Mar.) – 60%–100% (Apr.-Sept.); 100%–200% and over 200%,
respectively.</p>
        <p>As can be seen from Figure 2 and 3, the river flow maintains an ecological optimum
over the year, ranging from good to flushing, except for the period August–September,
during which the ecological status of rivers dramatically degrades to poor, leading to
damage of the river and riverside habitats.</p>
      </sec>
      <sec id="sec-3-2">
        <title>3.2 Classification of natural flow regimes for e-flow estimation</title>
        <p>
          The FDC combined with other methods, as Vogel and Fennessey (1995) indicated,
has been used in many hydrologic studies including flood control, water quality
management and aquatic habitats maintenance, due to its easy application and
expression of wealth hydrologic information
          <xref ref-type="bibr" rid="ref20 ref21 ref22 ref22 ref23 ref23 ref4 ref6">(Dakova et al., 2000; Smakhtin and
Anputhas, 2006; Smakhtin et al., 2006; Shaeri Karimi et al., 2012; Efstratiadis et al.,
2014; Ridolfi et al., 2020)</xref>
          .
        </p>
        <p>In an attempt to find a more comprehensive approach to determine environmental
flow requirements for the Rhodope Mountain transboundary area, FDCs were prepared
for all gauging stations based on long-term data (from 10 to 28 years), which is
sufficient to assess the availability of water in the study area. Focusing on duration of
low flow events, several indices, obtained from FDCs, were used for the purpose of
this study, which are most often employed in the government and academic literature
regarding environmental flow assessment (Table 2).</p>
        <p>
          Average flow magnitude (Q50), Q90 and Q95 exceedance flows overall years,
expressed as well in percentages of the MAF, were obtained directly from the FDCs.
Low exceedance flows index (LEFI) was calculated dividing mean magnitude of flows
exceeded 90% of the time (Q90) by Q50
          <xref ref-type="bibr" rid="ref15 ref16 ref2 ref3 ref5">(Clausen and Biggs, 1997, 2000)</xref>
          , while Base
flow variability index (BVI) was obtained dividing Q50 by Q90
          <xref ref-type="bibr" rid="ref17 ref19">(Nelms et al., 1997;
Pyrce, 2004)</xref>
          . Those indices combined with expert opinion were utilized to define
ecological management classes (EMC), which express the state of the riverine
ecosystems, based on the e-flow regimes, following the procedure proposed by
Smakhtin and Anputhas (2006) and some of the steps proposed by South African
Water Research Commission
          <xref ref-type="bibr" rid="ref11">(King et al. 2008)</xref>
          . The relationship between the
lowflow indices and the biological conditions of the benthic biota (e.g. elements such as
biomass, total number of species, etc.) was also taken into account
          <xref ref-type="bibr" rid="ref2 ref5">(Clausen and Biggs,
1997)</xref>
          . The five EMCs were predetermined (see Table 3) assuming that higher EMC
requires more water as a percentage of MAF with low baseflow variability for
ecosystem maintenance and conservation.
        </p>
      </sec>
      <sec id="sec-3-3">
        <title>Description of water, habitat and ecosystem quality</title>
        <p>Negligible modifications from natural conditions: Rivers with minor
changes in in-stream and riparian habitats. Negligible risk to intolerant
biota.</p>
        <p>Slight modifications from natural conditions: Ecologically important
rivers with largely intact biodiversity and habitats. Slight risk to intolerant
biota.</p>
        <p>Moderate modifications from natural conditions: The habitats and
dynamics of the biota have been disturbed, but basic ecosystem functions
are still intact. Moderate risk to intolerant biota.</p>
        <p>High degree of modifications from natural conditions: Large changes in
natural habitats, biota and basic ecosystem functions have occurred. Habitat
diversity and availability have declined. High risk of loss of intolerant
biota.</p>
        <p>Critical degree of modifications from natural conditions: Modifications
have reached a critical level and ecosystems have been completely
modified with almost total loss of natural habitats and biota.</p>
        <p>Q90 exceedance flow, expressed in percentages of MAF, was selected in order to
pre-define the spatial extend of the EMCs. The values for Q90, which vary from 32.8%
to 1.9% of MAF, were interpolated in the ArcGIS software and were classified into
five classes, corresponding to A, B, C, D and E of the EMC, respectively (Figure 4a).
The same procedure was applied to BVI, identifying five baseflow variability classes
(Figure 4b).</p>
        <p>Looking at the continuity of the FDCs distribution (Figure 5: a, b), the five classes
can be clustered into two groups of rivers: (1) rivers of high quality habitats (classes A
and B) with negligible to slight risk of degradation (15% of the total area) and (2) rivers
of low quality habitats (classes C, D and E) with moderate to high risk of degradation.
The second group differs significantly in the stability of the flow regime, expressed
through greater flow variability over time.</p>
        <p>This study is the first attempt for a comprehensive environmental flow assessment
in Bulgaria, where other ecological information (e.g. biological parameters) is still
scarce. The Bulgarian-Greek Rhodope Mountain Range was chosen for this purpose,
which is the most important transboundary area for both countries. A simple procedure
combining hydrological methods, a holistic approach and geoinformation techniques
was applied, emphasizing the spatiotemporal variability of the flow regime.</p>
        <p>The results indicate that to maintain the rudimentary functions of the rivers in the
Rhodope Mountain Range requires an average daily flow in the range of 0.027 to 6.11
m3/s with a probability to exceed 90% of the time, which varies from 1.92 to 32.98%
of the MAF. On the other hand, the base flow variability index changes between 2
(low) and 20 (extremely high). High resolution gridded surfaces were generated by
spatial interpolation of the obtained data. Based on the calculated indices and applying
expert judgment of the flow regime, the rivers were regionalized into 5 EMCs
according to their potential to maintain the whole riverine ecosystem.</p>
        <p>Finally, a disadvantage of this study is that it relies solely on hydrological data. We
therefore recommend building а more holistic methodology, involving biological
surveys and socio-economic information, which can better define the environmental
flow requirements in the transboundary region.</p>
        <p>Acknowledgments. Part of the present study was conducted during D. Myronidis
Erasmus+ Staff Mobility for training mission in the Space Research and Technology
Institute (SRTI) research unit of the Bulgarian Academy of Sciences (BAS) between
28/10–01 and 11/2019.</p>
      </sec>
    </sec>
  </body>
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