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        <article-title>Effects of Dietary Soybean Meal Substitution by Flaxseed and Lupins on Kefalograviera Cheese Chemical Composition - Abstract</article-title>
      </title-group>
      <contrib-group>
        <contrib contrib-type="author">
          <string-name>Ilias Giannenas</string-name>
          <xref ref-type="aff" rid="aff2">2</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Eleftherios Bonos</string-name>
          <xref ref-type="aff" rid="aff0">0</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Ioannis Skoufos</string-name>
          <email>jskoufos@uoi.gr</email>
          <xref ref-type="aff" rid="aff0">0</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Erasmia Sidiropoulou</string-name>
          <xref ref-type="aff" rid="aff2">2</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Katerina Grigoriadou</string-name>
          <xref ref-type="aff" rid="aff1">1</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Zoitsa Basdagianni</string-name>
          <xref ref-type="aff" rid="aff3">3</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Chrysoula Karaiskou</string-name>
          <xref ref-type="aff" rid="aff3">3</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Stylianos Vasilopoulos</string-name>
          <xref ref-type="aff" rid="aff2">2</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Alexandros Patsavouras</string-name>
          <xref ref-type="aff" rid="aff0">0</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Symeon Skaftouros</string-name>
          <xref ref-type="aff" rid="aff0">0</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Aikaterini Nelli</string-name>
          <xref ref-type="aff" rid="aff0">0</xref>
        </contrib>
        <contrib contrib-type="author">
          <string-name>Athina Tzora</string-name>
          <xref ref-type="aff" rid="aff0">0</xref>
        </contrib>
        <aff id="aff0">
          <label>0</label>
          <institution>Department of Agriculture, School of Agriculture, University of Ioannina</institution>
          ,
          <addr-line>Kostakioi Artas, Arta</addr-line>
          ,
          <country country="GR">Greece</country>
        </aff>
        <aff id="aff1">
          <label>1</label>
          <institution>Institute of Plant Breeding and Genetic Recourses, HAO-DEMETER</institution>
          ,
          <addr-line>Thessaloniki</addr-line>
          ,
          <country country="GR">Greece</country>
        </aff>
        <aff id="aff2">
          <label>2</label>
          <institution>Laboratory of Nutrition, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki</institution>
          ,
          <addr-line>Thessaloniki</addr-line>
          ,
          <country country="GR">Greece</country>
        </aff>
        <aff id="aff3">
          <label>3</label>
          <institution>School of Agriculture, Aristotle University of Thessaloniki</institution>
          ,
          <addr-line>Thessaloniki</addr-line>
          ,
          <country country="GR">Greece</country>
        </aff>
      </contrib-group>
      <fpage>584</fpage>
      <lpage>585</lpage>
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      <title>-</title>
      <p>Summary</p>
      <p>Recently, novel dietary strategies are under examination, aiming to improve the
sensory characteristic, as well as the potential health benefits of animal products, such
as cheeses produced by sheep milk. This study examined the possible changes in the
chemical composition and fatty acid profile of Greek kefalograviera cheese, caused by
the partial replacement of soybean meal by flaxseeds and lupins. Initially, 30 dairy
ewes (Lesvos and Chios crossbreed) were fed conventional diets, based on alfalfa hay,
straw and concentrate feed that contained soybean meal for a period of one month and
on day 30, milk samples were collected from all animals. Then, for a period of two
months the same ewes were fed a second concentrate feed with lower level of soybean
meal that contacted 10% flaxseed and 10% lupins. On days 60 and 90, milk samples
were collected from all animals. The milk from each collection was used to
manufacture three kefalograviera cheeses (A=30-day, B=60-day, C=90-day), using
commercial starter culture, rennet and pasteurized milk. After 3 months of ripening,
from each kefalograviera cheese samples were taken for chemical analysis
(FoodScanTM) and fatty acid analysis (gas chromatography method). Cheese A had the
highest fat content (A:30.5%; B: 25.6%; C:28.10%), and the lowest moisture content
(A:37.9%; B:42.4%; C:39.6%) and protein content (A:26.9%; B:27.1%; C:29.1%).
The fatty acid profile results showed that Cheeses B and C contained higher amounts
of polyunsaturated fatty acids compared to the control (A:3.63%; B:5.76%; C:7.59%),
such as linoleic acid (A:2.26%; B:3.08%; C:3.89%), alpha-linolenic acid (A:0.16%;
B:0.72%; C:1.23%) and gamma-linolenic acid (A:0.49%; B:0.94%; C:1.26%).
Moreover, cheeses B and C had improved omega-6/omega-3 ratio (A:18.66; B:5.75;
C:4.14). In conclusion, the substitution of soybean meal by flaxseed and lupins in ewes’
diets, can result in modified Kefalograviera cheese composition with improved fatty
acid profile and potential health benefits. Future work has to be done in relation to
standardize diets and omega-3/omega-6 profiles in Kefalograviera cheese.</p>
      <p>JEL Codes: N50; Q10; Q13.</p>
      <p>Acknowledgments. This research has been co-financed by Greece and the European
Union (European Regional Development Fund) in context “Research – Create –
Innovate” within the Operational Program (Competitiveness, Entrepreneurship and
Innovation (ΕΠΑΝΕΚ) of the NSRF 2014-2020. Project Code: Τ1ΕΔΚ-04727.
Acronym “GreenFeeds”.</p>
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