OGG-Bac: Ontological Representation of Orthologous Bacterial Genes MengLiu School of Life Sciences & Biotechnology State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong University
Shanghai China
Medical School University of Michigan
48109 Ann Arbor MI USA
Hong-YuOu hyou@sjtu.edu.cn. School of Life Sciences & Biotechnology State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong University
Shanghai China
YongqunHe yongqunh@med.umich.edu Medical School University of Michigan
48109 Ann Arbor MI USA
OGG-Bac: Ontological Representation of Orthologous Bacterial Genes 67F247A4BC93F9C85DBF5DF36AFF9828 GROBID - A machine learning software for extracting information from scholarly documents OGG ortholog bacteria gene

Different from eukaryotic organisms that turn to have one set of gene annotations, bacteria are usually annotated with strainspecific genome annotations within which a large number of genes (e.g., DNA replication initiation factor dnaA gene) may be orthologs. To systematically integrate and analyze bacterial genes, we developed a bacterial branch of the Ontology of Genes and Genomes (OGG-Bac) with a focus on standardized orthologous gene representation and annotations.
Introduction

Eukaryotic organisms usually have one set of gene annotations for each species (e.g., human). For bacteria, each bacterial species turns to have decades and even hundreds of strains with sequenced genomes, and each genome is individually annotated. Therefore, it is very difficult to integrate the huge number of bacterial genes in the same species or at higher taxonomy levels.

Orthologous genes have evolved from a common ancestral gene. Using the OGG (an OBO Foundry library ontology) as a platform, an ontological representation of the hierarchy and properties of the bacterial orthologs at different taxonomic levels can facilitate the standardization and better understanding of the complex relations among various bacterial genes.

Methods

OGG-Bac was aligned with the OGG-core. The web servers Ontobee (http://www.ontobee.org/) and Ontofox (http://ontofox.hegroup.org) were applied to check and import the related existing terms from reliable ontologies. The Proté gé -OWL editor was used for manually ontology editing. We are first representing the genes in the synthetic minimal bacterial genomes (1) and their orthologous genes in other bacteria.

Results
Conclusions

OGG-Bac was developed to ontologically represent and annotate bacterial genes based on the ortholog gene hierarchy at different taxonomical levels.

Figure 1Figure 1 uses gene dnaA to illustrate how OGG-Bac represents different levels of bacterial orthologs. The dnaA gene encodes the DNA replication initiation factor in bacteria. It is the first gene in the synthetic minimal bacterial genomes (1) and conserved in various bacteria (e.g., Brucella and E. coli).
Figure 1 .Figure 1. Representation of dnaA gene in OGG-Bacteria Our representation strategy principles: (i) Use unique gene IDs for different levels of dnaA. (ii) Strain-specific genes are asserted based on their taxonomy hierarchy. (iii) For annotation, use the most common gene name as the gene label, and includes other gene labels (if different) as the alternative labels (Figure 1).
Acknowledgments

National Key R&D Program of China [2017YFC1600100 to H.Y.O.]; ML was supported by a jointly funded Ph.D.studentship of the China Scholarship Council and University of Michigan Medical School (Grant No. 201806230209).

Design and synthesis of a minimal bacterial genome CAHutchison R.-YChuang VNNoskov NAssad-Garcia TJDeerinck MHEllisman JGill KKannan BJKaras LMa Science 351 6253 2016