Use of Aromatic Medicinal Plants By-products for the Development of Beverages with Beneficial Properties - Abstract Argyro Vontzalidou 1, Evanthia Dina 1, Katerina Grigoriadou 2, Panagiotis Bagatzounis 3, Styliani Alexandri 2, Georgia Koutouzidou 2, Ilias Giannenas 4 and Nektarios Aligiannis 1 1 Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens, Greece 2 Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization – DEMETER, P.O. Box 60458, 570 01 Thermi, Thessaloniki, Greece 3 M. Bagatzounis & Sons S.A, Vatero, 50100, Kozani, Greece 4 Laboratory of Nutrition, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece Summary 1 During the processing of cultivated aromatic medicinal plants for their commercial use, a great number of inferior b’-quality products and by-products is obtained, which although until now are treated as "waste", they are rich in bioactive natural products with beneficial properties on human health. After a bibliographical research about the uses, the biological properties and the chemical content of plant species with significant amount of by-products, a number of herbs was further investigated for the development of beverages with slimming and relaxing activity and for the stimulation of immune system. The selected species for their slimming properties were Camellia sinensis and Aloysia citrodora and for their relaxing properties Tilia cordata, Matricaria recutita, Melissa officinalis and Passiflora incarnata. Also, for the stimulation of immune system, Origanum dictamnus, Sideritis sp., Cistus creticus and Echinacea purpurea were chosen. Techniques of infusion and water decoction were used for the preparation of extracts, in different proportions of mixing plant materials and solvent, for the three beverage categories. The methanolic extracts were, also, prepared in order to characterize the quality of the raw materials and to correlate their composition with the properties of the final products. All the extracts derived from infusion and water decoction were evaluated for their chemical profile (HPTLC technique), antioxidant capacity (DPPH free radical scavenging method) and their total phenolic content (Folin–Ciocalteu method). Then, the different mixtures were evaluated for their organoleptic characteristics (smell, taste, colour) for the selection of the final plant materials to be used for the development of the final beverages. The technique of infusion was selected, compared with water decoction, as the most suitable for the preparation of all the final beverages. After tests for the improvement of taste and smell, the composition of the final products was as follows: The beverage with slimming activity is a mixture of Camellia sinensis and Aloysia citrodora (1:1) with the addition of maltodextrin in specific quantity, the beverage with relaxing properties is a mixture of Tilia cordata, Matricaria recutita, Melissa officinalis, Passiflora incarnata (1:1:1:1) and the beverage for the stimulation of immune system is a mixture of Sideritis sp., Origanum dictamnus, Cistus creticus, Echinacea purpurea (1.5:0.5:1:1). Hence, the preparation of the three beverages through the utilization of plant material by-products, that are not commercially accepted, highlighted to develop novel products with benefits for human health and economic value can through the exploitation of aromatic medicinal plants by-products. Proceedings of HAICTA 2022, September 22–25, 2022, Athens, Greece EMAIL: avontzalidou@pharm.uoa.gr (A. 1); evanthiadina@gmail.com (A. 2); grigokat@outlook.com (A. 3); panos@bagatzounis.com (A. 4); stelalexa@yahoo.gr (A. 5); koutouzidoug@gmail.com (A. 6); igiannenas@vet.auth.gr (A. 7); aligiannis@pharm.uoa.gr (A. 8) ORCID: 0000-0003-4270-5415 (A. 1); 0000-0002-8000-9982 (A. 3); 0000-0002-6077-0504 (A. 6); 0000-0003-3145-3734 (A. 7); 0000- 0002-3335-8135 (A. 8) ©️ 2022 Copyright for this paper by its authors. Use permitted under Creative Commons License Attribution 4.0 International (CC BY 4.0). CEUR Workshop Proceedings (CEUR-WS.org) 446 Keywords Aromatic medicinal plants, by-products, beverages Acknowledgements This research was co-financed by the European Regional Development Fund of the European Union and Greek national funds through the Operational Program Competitiveness, Entrepreneurship and Innovation, under the call RESEARCH-CREATE-INNOVATE (project code: T1EDK--05041, Acronym: FeedMAP). 447