=Paper= {{Paper |id=Vol-3293/paper98 |storemode=property |title=The Alleviation of Water Deficit via Biostimulant Application in Greenhouse Grown Lettuce Plants - Abstract |pdfUrl=https://ceur-ws.org/Vol-3293/paper98.pdf |volume=Vol-3293 |authors=Paraskevi Bourouni,Vasilis Roupas,Christina Chaski,Spyridon Petropoulos |dblpUrl=https://dblp.org/rec/conf/haicta/BourouniRCP22 }} ==The Alleviation of Water Deficit via Biostimulant Application in Greenhouse Grown Lettuce Plants - Abstract== https://ceur-ws.org/Vol-3293/paper98.pdf
The Alleviation of Water Deficit via Biostimulant Application in
Greenhouse Grown Lettuce Plants - Abstract
Paraskevi Bourouni 1, Vasilis Roupas 1, Christina Chaski 1 and Spyridon Petropoulos 1
1
    University of Thessaly, Argonafton & Filellinon, 38221 Volos, Greece


                 Summary 1
                 Water shortage is considered one of the most important limiting factors for cropping under arid
                 and semi-arid conditions. This problem is expected to worsen in the following years within the
                 scenario of the ongoing climate crisis as the result of the uneven distribution of rainfalls
                 throughout the year and the increasing periods of drought. Vegetable production is among the
                 most critical sectors of the primary production and water availability is essential for high yields
                 and high quality of produce. Therefore, water management through modern agronomic
                 practices is pivotal to ensure high yields and food security. The aim of the present study was
                 to evaluate the effect of deficit irrigation and biostimulant application on lettuce plants grown
                 directly in soil under protected environment. Two cultivars were selected (one Romaine and
                 one Batavia type), while young seedlings were transplanted directly in soil 7 weeks after
                 sowing in seed trays. Three irrigation treatments were applied based on the water field capacity
                 (FC) of soil, namely Control (100% FC), I1 (66% FC) and I2 (46 of field capacity). Moreover,
                 six biostimulant treatments were applied, namely Control (no biostimulants added), AG109
                 (seaweed and plant extracts and mcrominerals), AGR100 (humic and fulvic acids),
                 AGR111+112 (Si and Ca mobilization agent, Ca and Zn) AGR113 (Si) and AGR114 (mixture
                 of 20 L amino acids). The experimental layout was designed according to split-plot design,
                 using the irrigation treatment as the main plot and the biostimulant treatments as the subplot
                 for each of the tested cultivars. Irrigation was scheduled based on the readings of Delta-T PR2/4
                 profile probe (Delta-T Devices Ltd., Burwell, UK) and according to the target soil moisture
                 content. Biostimulants were applied manually with foliar (AGR109, AGR111+111 and
                 AGR113) or soil application (AGR110 and AGR114) three times throughout the growing
                 period, while all seedlings were immersed in the respective biostimulant solution before
                 transplantation. Chlorophyll content (SPAD index) and plant height were recorded at three
                 sampling dates (one week after each biostimulant application), while yield and growth
                 parameters (leaves number, leaves fresh and dry weight, Leaf Area Index (LAI) and Specific
                 Leaf Area (SLA)) were recorded at harvesting. Our results showed a varied effect of the tested
                 factors on chlorophyll content of leaves and plant height throughout the growing season for
                 both cultivars. Moreover, deficit irrigation at 46% FC resulted in a significant decrease of fresh
                 biomass, regardless of the cultivar and the biostimulant product. Interestingly, the mild water
                 shortage (irrigation 66% of field capacity) resulted in similar or higher yields compared to full
                 irrigation, especially in the case of AGR109, AGR110 and AGR 114 in Batavia lettuce and
                 AGR110 in Romaine lettuce. The highest yields in Batavia lettuce were recorded for AGR113
                 (315.4 g per plant) and AGR114 (317.9 g per plant) at 100% FC and 66% FC, respectively,
                 while AGR111+112 resulted in the highest yield at 46% FC. On the other hand, the highest
                 yield in Romaine lettuce was recorded for the control treatment (no biostimulants; 297.5 g per
                 plant) and AGR109 (292.6 g per plant) at 100% FC, while the same biostimulant (AGR109)
                 resulted in the highest yield at 66% FC. AGR113 treatment was the most productive at 465 FC
                 irrigation. A varied response to irrigation regime and biostimulant application was also
                 observed for the number of leaves, LAI and SLA values, although in most cases the application
                 of biostimulants alleviated the negative effects of water stress. In conclusion, the combinatory


Proceedings of HAICTA 2022, September 22–25, 2022, Athens, Greece
EMAIL: pbourouni@uth.gr (A. 1); vasilisroup@gmail.com (A. 2); xristina_xaski@hotmail.com (A. 3); spetropoulos@uth.gr (A. 4)
ORCID: 0000-0001-9292-1453 (A. 3); 0000-0002-0324-7960 (A. 4)
              ©️ 2022 Copyright for this paper by its authors.
              Use permitted under Creative Commons License Attribution 4.0 International (CC BY 4.0).
              CEUR Workshop Proceedings (CEUR-WS.org)




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application of mild water deficit and biostimulant showed promising results as an innovative
agronomic tool for water management.

Keywords
Lettuce, water deficit, biostimulant, fresh yield, water stress

Acknowledgements
This research has been co‐financed by the European Regional Development Fund of the
European Union and Greek national funds through the Operational Program Competitiveness,
Entrepreneurship and Innovation, under the call RESEARCH – CREATE – INNOVATE
(project code: T2EDK-05281). The APC was funded by the European Regional Development
Fund of the European Union and Greek national funds through the Operational Program
Competitiveness, Entrepreneurship and Innovation, under the call RESEARCH – CREATE –
INNOVATE (project code: T2EDK-05281).




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