These are the proceedings of the International Workshop on Petri Nets and Software Engineering (PNSE'2024) to be held at June 24 - 25, 2024. The workshop is co-located with 45th International Conference on Application and Theory of Petri Nets and Concurrency (PETRI NETS 2024) in Geneva, Switzerland. The workshop started in 1997 in Hamburg and emphasizes on the impact of software engineering in general and its development based on Petri nets. Therefore we have especially invited contributions that work on subjects that could be relevant for Petri nets, their application, supporting tools, etc. Results on other formalisms or semi-formal techniques are highly welcome. More information about the workshop can be found at: http://www.pnse.de/ This year we received 22 contributions for these proceedings. Each paper was reviewed by at least three referees. During the reviewing process the program comittee selected 17 contributions for publication. The workshop was organised in sessions with long and short presentations. The proceedings also contain the extended abstracts for the accepted poster contributions. We are glad that Wil van der Aalst accepted our invitation to give a talk about Lifting Process Discovery and Conformance Checking to the Next Level: A General Approach to Object-Centric Process Mining. The international program committee of PNSE'24 was supported by the valued work of Philippe Schnoebelen, Vladimir Bashkin, Marisol Barrientos, Johannes Lobbecke, Jakub Kovar, Beno^t Ries, Marc Schmidt, and Roman Nesterov as additional reviewers. Their valuable work is highly appreciated. Furthermore, we would like to thank our colleagues in the PC and the local organization team in Geneva for their support.