In follow-up to the four previous conferences, which were held in Utrecht, Groningen, Tilburg and Ravenstein, the fifth edition of the SIKS/BENAIS Conference on Enterprise Information Systems (EIS 2010) is held in Eindhoven this year. The purpose of the conference series is to bring together Dutch and Belgian researchers interested in the advances in and the business applications of information systems. Against that backdrop, we are happy to have received contributions from almost all of the Dutch and Belgian research groups active in this field.